AN INACTIVE PRECURSOR OF STREPTOCOCCAL PROTEINASE
نویسندگان
چکیده
منابع مشابه
An Inactive Precursor of Streptococcal Proteinase
1. Streptococcal proteinase is derived from an inactive precursor found in culture filtrates of proteinase-producing streptococci. 2. The precursor can be converted into the proteinase by low concentrations of trypsin but not by chymotrypsin. 3. In cultures grown in suitable media the conversion of precursor to proteinase is effected autocatalytically. This reaction occurs under reducing condit...
متن کاملAn Inactive Precursor of Streptococcal Proteinase* by Stuart
In a previous report (1) it was shown that, under suitable conditions, some strains of group A streptococci produce in broth cultures an extracellular proteolytic enzyme. In certain respects the enzyme resembles papain and the cathepsins in that it achieves maximal activity under reducing conditions and is inactivated by iodoacetic acid. In broth cultures the reducing conditions necessary for t...
متن کاملAn Electrophoretic Study of a Streptococcal Proteinase and Its Precursor
An electrophoretic study of crystalline preparations of a streptococcal proteinase and its precursor established their isoelectric points at pH values of 8.42 and 7.35 respectively (ionic strength 0.10). Preparations of the proteinase appeared to be electrophoretically homogeneous over a pH range of 5 to 8.5. Precursor preparations contained a relatively low concentration of the active enzyme v...
متن کاملAn Unusual Disulfide Bond in Streptococcal Proteinase.
The proteinase produced by Group A hemolytic streptococci has the unique property of containing only a single half-cystinyl residue per molecule (Liu, Neumann, Elliott, Moore, and Stein (1)). The -SH group of the potential cysteinyl residue is liberated only when the enzyme is activated by reduction. These findings raise the question as to the nature of the linkage in which the half-cystinyl re...
متن کاملThe Crystallization and Serological Differentiation of a Streptococcal Proteinase and Its Precursor
Grown in dialysate broth at a pH between 5.5 and 6.5, some strains of group A streptococci elaborate the precursor of a proteolytic enzyme. Within this range of hydrogen concentration the precursor is also produced when the streptococci are suspended in a peptone dialysate containing glucose and incubated at 37 degrees C. The precursor does not appear to be produced at a neutral or alkaline rea...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1947
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.85.3.305